Activated Alkynes-based Diverse Level Conjugation Technology

Bioconjugation plays important role in biomedicine. Although researchers have spent a lot of effort to promote bioconjugation chemistry forward, currently, available strategies have some limitations that restrict their applications in biology and biomedicine. Activated alkynes-based diverse level conjugation technology is born in this context. Creative Biolabs has focused on cell surface conjugation for years. Based on experience accumulated from practice, we are confident in providing customer-satisfied activated alkynes-based diverse level conjugation services to global customers.

Overview

During the last three decades, cell membrane engineering has provided an invaluable toolbox to regulate the molecular and biochemical composition of the cellular interface and investigate the processes governed by the plasma membrane components, such as signal transduction, endocytosis, adhesion, migration, and cell-cell interactions. These approaches have expanded beyond fundamental research into more practical applications, including drug delivery, cell encapsulation, or biosensing/recognition, as well as to study cellular aggregation processes and the formation of tissue-like organized structures. Bioconjugation plays an important role in cell membrane engineering and drives the great development of material science as well as biomedicine.

Activated Alkynes-based Diverse Level Conjugation Technology

Activated alkynes-based diverse level conjugation technology is a method that realizes bioconjugation at diverse levels through metal-free click reactions between optimized activated alkynes and the native groups. Activated alkynes-based diverse level conjugation technology can realize metal-free on-demand click bioconjugation. The bioconjugation can be diverse, for example, peptides, proteins, synthetic biofunctional polymers, and natural polysaccharides are all feasible for this method. Briefly, activated alkynes-based diverse level conjugation technology is expected to expand the bioconjugation methodology. This method has great potential of serving biomedicine and material science in the future.

Fig.1 Schematic illustration for the metal-free click bioconjugation with native groups of biotargets based on activated alkynes.

Advantages of the Technology

• Metal-free

With a metal-free strategy, this method has great potential in the application in biology and medicine.

• Facile labeling and functionalization

In this strategy, amine, thiol, and hydroxyl groups all can be used for labeling and functionalization. Utilization of these abundant native groups in bio targets as the substrate for labeling and functionalization will avoid the complexity and tedious premodification.

• Extensive biological applications:

Based on the characteristics of the technology, this method can be applied at the molecular level for the biofunctionalization of synthetic polymers, PEGylation, and labeling of peptides and proteins. In addition, at the cell level, the technology can also be applied in fast whole-cell mapping, and quick differentiation and labeling of Gram-positive bacteria.

Services at Creative Biolabs

With rich experience and advanced facilities, Creative Biolabs is capable of providing high-quality activated alkynes-based diverse level conjugation services. Besides strong experimental foundations, our excellent expert team will also help with every question during the project.

As an industry-leading CRO company, Creative Biolabs is committed to offering customer-satisfied services to every client. Our powerful technical background will assure the quality of the services. If you are interested in activated alkynes-based diverse level conjugation or any other services we provide, please don’t hesitate to contact us for more information.