Cell-Surface Anchor Engineering Technology

Cell-surface anchor engineering technology is a recent advance in biomolecular engineering via external integration of compounds such as synthetic protein complexes to mimic immune cell interaction and stimulation. The technology is important in the analysis of complex cell-surface interactions, the engineering of antigen presenting cells, the development of cancer vaccines, and possibly the protection against graft rejection. Creative Biolabs is committed to the development, transformation and promotion of breakthrough cell-surface anchor engineering technology for our global customers.

Cell-Surface Anchor Engineering and Advantages

Cell surface anchor engineering is an innovative biotechnology for construction. The cell surface is an attractive location for heterologous gene expression. Proteins localized on the cell surface play an important role in signal transduction, recognition, and transportation of environmental substances, morphogenesis, and various other reactions. In cell anchor surface engineering, functional heterologous proteins/drugs are genetically immobilized on the cell surface by fusion with the domains for cell wall- or plasma membrane-anchoring. And immobilization on the cell surface itself retains the features of enzymes, and increases the structural stability of the displayed proteins/drugs. The displayed proteins/drugs can interact with environmental substances with high-molecular mass that cannot be transported into a cell.

Fig.1 Schematic diagram of generation of cell-surface anchor engineered T cells. (Hao, 2020)

Case Study of Cell-Surface Anchor Engineering

• Case 1

A specific method by embedding DNA-origami nanodevices onto the cell surface can engineer cell-membrane function. It is anticipated that integration of DNA-origami nanodevices can transform the cell membrane into an engineered material to mimic and measure the biophysical and biochemical function of living cells.

• Case 2

The liposomal avasimibe was clicked onto the T cell surface by lipid insertion. Avasimibe could be restrained on the T cell surface during circulation and extravasation and locally released. Treatment with surface anchor-engineered T cells resulted in superior antitumor efficacy in mouse models of melanoma and glioblastoma.

• Case 3

Glycoinositol phospholipids (GPIs) serve as membrane anchors for numerous cell-surface proteins. These phospholipid-like anchors use a common structure as a general mechanism for membrane attachment irrespective of protein function.

Cell-Surface Anchor Engineering Technology Services

With years of experience in cell-surface engineering technology, Creative Biolabs is dedicated to constructing whole-cell biocatalysts by cell surface engineering, for applications in the bioproduction of chemicals, bioadsorption of heavy metals and rare metals, attractive, and biofuel production. Based on your project needs, Creative Biolabs provides the best-quality services in cell-surface engineering technology. Please contact us for more detailed information to know how we can help.

Fig.2 Various applications of cell surface engineering. (Ueda, 2016)

References

1. Hao, M.; et al. Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy. Science Translational Medicine. 2020, 12(571):eaaz6667.
2. Ueda, M. Establishment of cell surface engineering and its development. Bioscience Biotechnology and Biochemistry. 2016, 80(7):1-11.