Inquiry

2-1.gif

Cell-Antibody Conjugate (CAC) Service

Cell-based therapies have revealed distinctive therapeutic potential in the field of cancer immunotherapy. Therefore, modifying cells and manipulating their functions to achieve the desired treatment has become a major interest in the field of biomedical research. Although CAR-T based genetic modification is a powerful tool, its application is limited due to the permanent modification of cells. Alternatively, Creative Biolabs has developed an innovative method - CellFace™ conjugate technology, the most advanced cell surface engineering technology. This fast and simple technology allows for a stable conjugation of any biomolecule, especially macromolecules (proteins, antibodies) on the cell surface, while maintaining the cell viability.

img2-1-arrow.png img2-1-01.png img2-1-02.png img2-1-03.png img2-1-04.png

End-to-End CAC Service

01

Design

Cell Lines

Creative Biolabs offers hundreds of quality-controlled cell lines, including cancer cell lines with different disease states, immune cell lines, stem cells from multiple sources, and control cell lines. All cell lines have been validated and are free from mycoplasma, and undesirable cells and foreign factors such as bacteria, fungi, viruses. Before your project launches, you can consult our scientists and they will help you determine which cell line is best for your research.

Available cell lines

img2-1-07.png

Immmune Cells

img2-1-08.png

Stem Cells

img2-1-09.png

Disease State Cells

T cells Embryonic & Induced Pluripotent Stem Cells Bone Marrow Mononuclear Cells (BMMCs)
B cells Hematopoietic Stem Cells Rheumatoid Arthritis and Osteoarthritis Fibroblasts
NK cells Mesenchymal Stem Cells Lung Bronchial Epithelial Cells Hepatocytes
Dendritic cells Neural Stem Cells Hepatocytes
Monocytes Cancer Stem Cells Breast Cancer Cells
Neutrophils ... Hematopoietic Malignancies Cells
... Renal Cells
...

Antibodies

For antibody product, Creative Biolabs also provides professional custom antibody engineering services. Multiple antibody format are avalible, including: Fab, scFv, tribody, bispecific antibody, VHH, minibody, etc. In additon, we also provide antibody labeling service for further CAC analysis.

img-1-1-01.png

02

Conjugation

Conjugate antibody to cell surface in minutes

Based on our CellFace™ conjugate technology platform, we utilize fucosyltransferases (Fuc T) to transfer antibodies to the surface of living cells in minutes. This one-step approach developed by scientist team is a fast, simple, and cost-effective technique for cell surface engineering. Using this method, any antibody formats can be introduced to the cell surface without genetic modification of the host cells.

Notably, in addtion to antibody protien conjugates, other proteins can also be introduced to the cell surface including but not limit to:

  • Antigens
  • Hormones
  • Growth factors
  • Vitamins
  • Colony-stimulating factors
2-1.gif
img2-1-06.png

03

Analysis

Analyze antibody labeling efficiency and anti-tumor efficacy in vitro and in vivo

Creative Biolabs provides a full set of cell-antibody conjugate characterization services including:

Antibody Labeling Efficiency Analysis

We use confocal microscope to examine the surface labeling efficiency of antibodies. This high-resolution instrument has the ability to image specific cell types and can be used with a variety of fluorescently conjugated antibodies, making it very attractive for antibody localization.

2-1-Cell-Antibody-Conjugate-CAC-Serivice-1.png Fig.1 Confocal microscopy images of cells treated with or without FucT when incubated with IgG.

Antibody-dependent Cellular Cytotoxicity (ADCC) Analysis

One of the important mechanisms for antibody drugs to kill target tumor cells is antibody-dependent cell-mediated cytotoxicity (ADCC). To determine the CAC activity against specified target cell lines, Creative Biolabs performs ADCC assays to characterize target cell lysis in vivo and in vitro. The lysis of target cells is assesed through:

  • LDH (lactate dehydrogenase) assay
  • FACS screening
  • ATP release

To learn about Creative Biolabs' capabilities in cell-surface modification development, please don't hesitate to contact us.

Antibody - Antigen Binding Analysis

With the help of experienced scientists, our antibody binding assays is performed based on the following techniques:

  • Conventional ELISA
  • Flow cytometryg
  • Surface Plasmon Resonance (SPR)/ Octet Biolayer Interferometry (BLI) (recommended): give real-time unlabeled full kinetic analysis, and able to report affinity constants (KD) associated with detailed or "on" rates (Ka), dissociation rates (Kd) and/or "closed" rates.
img2-1-11.png
img2-1-12.png
img2-1-13.png

Types of Antibody Conjugates

ScFv Antibody
Fab Antibody
VHH Antibody
Bispecific Antibody
Minibody

All services are only provided for research purposes and Not for clinical use.

ONLINE INQUIRY

  • *
  • *
  • *
USA

United Kingdom