End-to-End CAC Service

01

Design

Cell Lines

Creative Biolabs offers hundreds of quality-controlled cell lines, including cancer cell lines with different disease states, immune cell lines, stem cells from multiple sources, and control cell lines. All cell lines have been validated and are free from mycoplasma, and undesirable cells and foreign factors such as bacteria, fungi, viruses. Before your project launches, you can consult our scientists and they will help you determine which cell line is best for your research.

Available cell lines

Immmune Cells	Stem Cells	Disease State Cells
T cells	Embryonic & Induced Pluripotent Stem Cells	Bone Marrow Mononuclear Cells (BMMCs)
B cells	Hematopoietic Stem Cells	Rheumatoid Arthritis and Osteoarthritis Fibroblasts
NK cells	Mesenchymal Stem Cells	Lung Bronchial Epithelial Cells Hepatocytes
Dendritic cells	Neural Stem Cells	Hepatocytes
Monocytes	Cancer Stem Cells	Breast Cancer Cells
Neutrophils	...	Hematopoietic Malignancies Cells
...		Renal Cells
		...

Antibodies

For antibody product, Creative Biolabs also provides professional custom antibody engineering services. Multiple antibody format are avalible, including: Fab, scFv, bispecific antibody, VHH, minibody, etc. In additon, we also provide antibody labeling service for further CAC analysis.

02

Conjugation

Conjugate antibody to cell surface in minutes

Based on our CellFace™ conjugate technology platform, we utilize fucosyltransferases (Fuc T) to transfer antibodies to the surface of living cells in minutes. This one-step approach developed by scientist team is a fast, simple, and cost-effective technique for cell surface engineering. Using this method, any antibody formats can be introduced to the cell surface without genetic modification of the host cells.

Notably, in addtion to antibody protien conjugates, other proteins can also be introduced to the cell surface including but not limit to：

• Antigens
• Hormones
• Growth factors
• Vitamins
• Colony-stimulating factors

03

Analysis

Analyze antibody labeling efficiency and anti-tumor efficacy in vitro and in vivo

Creative Biolabs provides a full set of cell-antibody conjugate characterization services including:

Antibody Labeling Efficiency Analysis

We use confocal microscope to examine the surface labeling efficiency of antibodies. This high-resolution instrument has the ability to image specific cell types and can be used with a variety of fluorescently conjugated antibodies, making it very attractive for antibody localization.

Fig.1 Confocal microscopy images of cells treated with or without FucT when incubated with IgG.

Antibody-dependent Cellular Cytotoxicity (ADCC) Analysis

One of the important mechanisms for antibody drugs to kill target tumor cells is antibody-dependent cell-mediated cytotoxicity (ADCC). To determine the CAC activity against specified target cell lines, Creative Biolabs performs ADCC assays to characterize target cell lysis in vivo and in vitro. The lysis of target cells is assesed through:

• LDH (lactate dehydrogenase) assay
• FACS screening
• ATP release

To learn about Creative Biolabs' capabilities in cell-surface modification development, please don't hesitate to contact us.

Antibody - Antigen Binding Analysis

With the help of experienced scientists, our antibody binding assays is performed based on the following techniques:

• Conventional ELISA
• Flow cytometryg
• Surface Plasmon Resonance (SPR)/ Octet Biolayer Interferometry (BLI) (recommended): give real-time unlabeled full kinetic analysis, and able to report affinity constants (KD) associated with detailed or "on" rates (Ka), dissociation rates (Kd) and/or "closed" rates.