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Cell-Fucose Conjugate Service

Fucosylation is a biological process widely observed in vertebrates, invertebrates, plants, bacteria, and fungi. The fucose moiety on the cell surface glycans is increasingly considered to be essential for cell-to-cell interactions and signaling processes. Studies have shown that immune cell-surface fucosylation leads to a result of improved homing and trafficking, and these benefits are being discussed in phase 1 clinical trials.

Scientists at Creative Biolabs are committed to providing the best-quality engineered fucosylated cell products. Benefiting from our proprietary cell surface modification technology, this fucose conjugate process is fast, simple, and cost-effective.

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One-Stop Cell-Fucose Conjugate (CFC) Service

Based on our CellFace™ conjugate technology platform, we utilize fucosyltransferases (Fuc T) to transfer fucose to the surface of living cells in minutes. Succesfully conjugated cells are characterizated by a full set of services including but not limit to:

  • Fucosylation analysis
  • CFC viability analysis
  • CFC migration and invasion assay
  • CFC Cytotoxicity Assay
  • CFC phenotypic analysis
  • Immune synapse studies
  • Binding assay
  • Cell imaging in vivo / in vitro (luciferase reporter)

Rational for Cell-surface Fucosylation

Multiple published data support the rational for cell surface modification.

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01

Inhibition of fucosylation by 2FF suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation

One of the most important glycosylation events in liver cancer progression is fucosylation. In this study, scientists used the L-fucose analog 2-fluoro-L-fucose (2FF) to interfere with the normal synthesis of GDP-fucose, and verified its potential effect in HepG2 liver cancer cell line. The following data clearly illustrate the effects of fucosylation on liver cancer growth in vivo. For further details, please click: https://www.nature.com/articles/s41598-017-11911-9#Sec9.

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Conjugation

Fucosylation of human regulatory T cells (Tregs) enhances resistance to graft-versus-host disease

This study demonstrates that by adding fucose to human Tregs, fucosylated Tregs show prolonged in vivo persistence in a heterogeneous GVHD mouse model. Compared to untreated Treg, mice given lower doses of fucosylated Treg had improved survival (70% to 30%; P <.0001). These preclinical data suggest that fucosylated human Tregs are an effective strategy to prevent GVHD. For more details, please click: https://ashpublications.org/blood/article-lookup/doi/10.1182/blood-2014-10-603449.

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Analysis

Fucosylation: From cellular adhesion to immune regulation

In this study, scientists discussed the function of fucosylation from cell adhesion to immune regulation. The role of fucosylation in the development of a variety of diseases including sickle cell disease, rheumatoid arthritis, tumor suppression, and metastasis prevention were also evaluated. The results below clearly illustrate fucosylation and immune cell development and plasticity. For more details, please click: https://www.cell.com/cell-chemical-biology/comments/S2451-9456(18)30066-7#secsectitle0010.

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For more details about our cell-fucose conjugate service, please feel free to contact us anytime.

All services are only provided for research purposes and Not for clinical use.

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