Cell surface modification is playing a more and more critical role in many biological types of research. However, in cell surface modification, it is necessary to ensure that the original composition and functionality of the cell will not be significantly changed or disturbed. At this point, polyvalent functionalization meets the demand. Creative Biolabs is an experienced cell surface engineering service provider. We have developed a comprehensive live-cell surface conjugation platform and will provide customer-satisfied cell surface-based services including polyvalent display conjugation to global clients.
The valency of a particle—a small molecule, oligosaccharide, protein, nucleic acid, lipid or aggregate of these molecules; a membrane or organelle; a virus, bacterium, or cell—is the number of separate connections of the same kind that it can form with other particles through ligand-receptor interactions. Polyvalent engineering is a method for the polyvalent surface display of biomolecules on live cells that could tremendously strengthen molecular recognition and holds the potential for broad biological and biomedical applications.
Fig.1 Graphical abstract of live cell-surface based polyvalent display conjugate technology.
Each DNA initiator has the ability to grow into a supramolecular DNA polymer with multiple branches, thus a small amount of DNA can realize the cell surface polyvalent modification. In addition, nucleic acids can be conjugated to many substrates such as micro-scale materials, nano-scale materials, and a diverse array of molecules. Thus, the nucleic acid is a powerful tool in cell surface polyvalent conjugation.
To realize the live-cell surface-based polyvalent display, the cells will experience two-step surface treatment under physiological conditions. In the first surface treatment, a DNA initiator (DI) will be displayed on the cells. During this process, to maintain high cell viability, a low concentration of DI is necessary. In the second surface treatment, researchers have demonstrated that DNA hairpin monomers (DMs) which have been rationally designed can polymerize in the presence of DI via a hybridization chain reaction. According to this result, the DI-displaying cells were reacted with two DMs to achieve polyvalent surface engineering in this treatment process.
As a professional cell surface conjugation service provider, Creative Biolabs provide reliable live cell surface-based polyvalent display conjugate services. Besides, we have gradually established our own technology platform containing a variety of advanced practicable, and significant technologies. Our services including but not limited to:
With professional expertise and strong foundations, Creative Biolabs aspires to bring every client top-rated customer experience. If you are interested on live cell surface-based polyvalent display conjugate services or any other technologies in our website, please don't hesitate to contact us for more information.
All services are only provided for research purposes and Not for clinical use.