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Cell-Fluorescent Protein Conjugate Service

With the advent of advanced optical imaging technology, understanding of the regulation and function of cell surface proteins has rapidly progressed. Gaining a comprehensive understanding of cell surface protein regulation mechanisms and their functions, it is necessary to monitor its interaction with surrounding cell membrane components (such as other proteins and lipids) and cell-to-cell interactions. Creative Biolabs has developed a 'one-step' cell-fluorescent conjugate technology that allows for observing cell structure and processes in real time and over time. This technology delivers more reliable and relevant information than traditional fluorescent labeling technology.

Design

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Conjugation

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Imaging and Analysis

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One-Stop Cell-Fluorescent Protein Conjugate Service

Whether you are new to cell imaging or want to confirm your knowledge, Creative Biolabs is capable of leveraging our best expertise to advance your research. Our one-stop cell-fluorescent protein conjugate servic ensures to deliver relible cell imaging and trackig information!

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01

Design

Cell line and fluorescent protein preparation

Fluorescent Protein Preparation

Creative Biolabs conjugates a broad group of classic fluorescent markers, including fluorescein, rhodamine, Texas red, CyDyes™, and phycoerythrin (RPE). All fluorescent conjugates are optimized for excellent absorption (high extinction coefficient), high fluorescence quantum yield and excellent high photostability. Choosing the right fluorescent protein depends on your experimental purpose and available excitation wavelengths in your lab. If you still have questions, just consult our technical team for help.

The table below summarizes the fluorescent dyes available at Creative Biolabs:

Fluorescent Dyes

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Conjugation

'One-step In Minutes'

Based on our CellFace™ conjugate technology platform, we utilize fucosyltransferases (Fuc T) to transfer fluorescent protein to the surface of living cells in minutes. This one-step approach developed by scientist team is a fast, simple, and cost-effective technique for cell surface engineering. In this way, any fluorescein protein can be tightly introduced onto the cell surface without affecting host cell activity.

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Imaging and Analysis

Creative Biolabs provides a full set of CFPC characterization services including:

CFPC Characterization

  • Fluorescence-to-cell ratio (FCR) assessment
  • Photostability assessment
  • Phototoxicity optimizaiton
  • Live cell localization
  • Live cell tracking
  • Cell viability analysis
  • Environmental control for long-term imaging or senstive cells
  • Other QC analysis available upon request
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Linker-Payload Characterization

  • Potencyy
  • Solubilityg
  • Plasma and PH stability
  • Payload release kineticse
  • Purity, chemical stability and other analytical properties uopon request
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Applications of CFC

3D imaging of live cells
3D imaging of tissues, model organisms
cell-cell interactions
cell tracking
biosensing
cell shape

All services are only provided for research purposes and Not for clinical use.

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