Connect with Us at the Upcoming Events

Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at the 13th Annual World ADC San Diego and the 13th Annual World Multispecific Summit in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting


Live Cell Surface-based N-Glycan Editing Technology

Mammalian proteins are highly glycosylated, especially on cell surfaces. Cell-surface glycans are involved in a number of biological functions. Thus, the importance of live cell surface-based N-glycan editing is self-evident. Focusing on cell surface engineering over years, Creative Biolabs has accumulated extensive experience from practice. With rich experience and strong foundations, we are capable of offering high-quality live-cell surface-based N-glycan editing services to global customers.

Diagram of cell surface glycans Fig.1 Diagram of cell surface glycans. (Briard, 2018)

Introduction of N-Glycan

Mammalian proteins are highly glycosylated, and protein glycosylation, the most common post-translation modification, is involved in many biological events, especially on cell surfaces. Cell-surface glycosylation involves N-glycans, O-glycans, glycosylphosphatidylinositol anchors, glycosaminoglycans, glycolipids, and so on. N-glycans (the main form of protein glycosylation) comprise various structural subtypes, including high-mannose, complex, hybrid, core-fucosylated, non-core-fucosylated, and bisecting types, among others. Cell-surface glycans are involved in a number of biological functions, including cell-cell communication, membrane protein trafficking, pathogen invasion, and immune response. The pursuit of well-defined N-glycosylation at the cellular level requires a constructive strategy.

Methods of Live Cell Surface-based N-Glycan Editing

Researchers have discovered a series of CHO N-glycosylation mutants with lectin resistances, in which glycosyltransferase levels change and affect N-glycan biosynthesis, producing N-glycan structures with less heterogeneity. A similar concept of glycosyltransferase engineering has been applied in the glycoengineering of therapeutic glycoproteins. In a FUT8 knockout CHO cell line or a glycoengineered Pichia pastoris expression system, immunoglobulin-G (IgG) antibodies bearing optimized N-glycan structures were produced with enhanced anti-tumor efficacy. This strategy provided a useful tool to control the N-glycosylation of secreted proteins or membrane proteins.

Selective editing and labeling of specific monosaccharides on cell surfaces via chemical biology techniques, such as metabolic glycan labeling, selective oxidation, and glycosyltransferase-catalyzed modification, has gradually been established. Selective oxidation of monosaccharides with a chemical oxidant or oxidase provides an alternate strategy to label cell-surface glycans. By chemical biology techniques, many remarkable works achieve cell-surface glycan labeling and living cell imaging, focusing on the selective detection of monosaccharides or disaccharides.

ENGase-catalyzed N-glycosylation remodeling is an emerging technique for the preparation of homogeneous glycoproteins that have been developed substantially over the past decade. Wild-type (WT) ENGases cleave the GlcNAcβ1,4GlcNAc motif of the N-glycan core and remove native heterogeneous glycoforms, then the ENGase mutants reassemble a homogeneous synthetic N-glycan substrate onto the pretreated glycoprotein carrying the innermost GlcNAc. The application of this method in Lec4 CHO mutant cells achieved a less-heterogeneous-to-homogeneous strategy of glycan editing, which will be greatly beneficial for the exploration of the precise glycan-associated functions of membrane glycoproteins.

Services at Creative Biolabs

As a global leading CRO company, Creative Biolabs has rich experience in cell surface engineering and has accomplished hundreds of relevant projects. Based on these conditions, we are capable of providing high-quality live-cell surface-based N-glycan editing services.

Creative Biolabs is eager to bring every client a top-rated customer experience. If you are interested in live-cell surface-based N-glycan editing services or any other services on our website, please don't hesitate to contact us for more information.


  1. Briard, J. G.; et al. Cell-based glycan arrays for probing glycan-glycan binding protein interactions. Nat Commun. 2018, 9(1): 880.

All services are only provided for research purposes and Not for clinical use.


  • *
  • *
  • *

United Kingdom