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Cell Surface-based Biotinylation Modification Service

The high-affinity biotin-avidin interaction is highly resistant to some harsh denaturing conditions, including heat, pH, and organic solvents. Hence, biotinylation has promising applications in cell surface engineering. With years of experience in the research of cell surface engineering, Creative Biolabs is capable of offering high-quality cell surface-based biotinylation modification services to global clients.

Introduction of Cell Surface-based Biotinylation Modification

The process of biotinylation is a covalent attachment of biotin molecule, a water-soluble vitamin B7, to cell surfaces, biomaterials, small molecules, and macromolecules. The high-affinity biotin-avidin (as well as avidin analogs streptavidin and NeutrAvidin) interaction is highly resistant to heat, pH, organic solvents, and other denaturing environments and as a result, has promising applications in different biotechnological and biomedical fields. Researchers have taken advantage of this extremely high-affinity binding between biotin and its binding partners to expand the tool kit for cell surface modification. Commercially available heterobifunctional biotin terminated linkers which are chemoselective for amines, thiols, and carbonyl groups are the most commonly used reagents for biotinylation of the cell surface. In some instances, enzymes such as biotin ligase can also be used as a biotinylation agent. Once the cell is labeled with biotin, it can be readily functionalized with a wide range of tagged small molecules, microspheres, polymers, or proteins through an avidin bridge.

Methods and Applications

Schematic of the endothelial surface grafting with microspheres through the biotin-avidin bridge. Fig.1 Schematic of the endothelial surface grafting with microspheres through the biotin-avidin bridge.(Abbina, 2018)

Engineering the MSCs surface using the conjugation of SLeX by covalent biotinylation and a streptavidin-biotin bridge to improve their rolling interactions in vitro. Fig.2 Engineering the MSCs surface using the conjugation of SLeX by covalent biotinylation and a streptavidin-biotin bridge to improve their rolling interactions in vitro. (Abbina, 2018)

Services at Creative Biolabs

Committed to cell surface engineering research for years, Creative Biolabs has accumulated rich experience from hundreds of successful precedents. We have established an advanced platform with comprehensive technologies and facilities. In addition, our scientists with advanced degrees will be responsible for every step of the projects.

As a professional cell surface engineering services provider with a great industrial reputation, Creative Biolabs will be your best partner. If you are interested in cell surface-based biotinylation modification services or other cell surface conjugation services, please don't hesitate to contact us for more information.

Reference

  1. Abbina, S.; et al. Surface engineering for cell-based therapies: Techniques for manipulating mammalian cell surfaces. ACS Biomater Sci Eng. 2018, 4(11): 3658-3677.

All services are only provided for research purposes and Not for clinical use.

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