Cell Surface-based Lipid Membrane Fusion Service

Lipid membrane fusion has some critical roles in many important cellular processes (such as phagocytosis and exocytosis) and has been intensely investigated for cell surface engineering. Focusing on cell surface engineering over years, Creative Biolabs has grown into an excellent and professional cell surface-based modification services provider. With extensive expertise and experience, we are confident in providing global clients with high-quality cell surface-based lipid membrane fusion services.

Introduction of Cell Surface-based Lipid Membrane Fusion

Lipid membrane fusion is mediated by a spontaneous lipid leaflet fusion process, which generally occurs between two distinct lipid bilayer-directed structures (such as the liposome and cell membrane). Liposome fusion is a special case of hydrophobic insertion used to deliver a large number of non-endogenous substrates to the cell surface. In this process, lipid vesicles composed of phospholipids containing unique functionalities dock onto the cell surface and are subsequently triggered to fuse with the cell membrane. This process mimics pre-existing cellular processes in which contents encapsulated by lipid vesicles are delivered to tissues via membrane fusion.

Fig.1 A representative scheme of cell surface engineering based on liposome fusion. (Abbina, 2018)

Application in Regenerative Medicine

The generation of complex three-dimensional (3D) tissues with multiple cell types in vitro is a major area of research in regenerative medicine dedicated to the in vitro generation of artificial organs. The proper functioning of organs is extremely sensitive to minor changes in tissue structure and organization. As such, control over cellular assembly would be a huge advantage in streamlining the generation of artificial organs. Researchers have demonstrated such control by using liposome fusion mediated click chemistry (ketone-oxime) to construct complex cardiac tissue composed of cardiomyocytes, cardiac fibroblasts, and human umbilical vein endothelial cells (HUVECs). Ketone and oxyamine presenting cells were rapidly clicked together via the stable oxime ligation and assembled into spheroids and then tissues. When added in sequential order, assembled tissues displayed defined layers allowing for strict pattern control. The various tissue constructs were stable and viable for several weeks.

Fig.2 Bio-orthogonal assembly of 3D cardiac tissue. (Abbina, 2018)

Services at Creative Biolabs

Creative Biolabs has been focusing on cell surface engineering research for a long time during which we have accomplished hundreds of customer-satisfied projects. We have accumulated rich experience and gradually developed a comprehensive technology platform. Our platform has advanced facilities and professional specialists as strong guarantees for the quality of our services.

With rich working experience in the field of cell surface engineering, Creative Biolabs is eager to bring our high-quality services to global researchers holding need. If you are interested in cell surface-based lipid membrane fusion services or you have any other questions, please don't hesitate to contact us for more information.

Reference

1. Abbina, S.; et al. Surface engineering for cell-based therapies: Techniques for manipulating mammalian cell surfaces. ACS Biomater Sci Eng. 2018, 4(11): 3658-3677.