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Cell Surface-based Metabolic Conjugation Service

Working together, chemists and biologists will be able to develop methods that combine genetic and metabolic engineering to introduce a range of unnatural epitopes onto cells without the restrictions imposed by nature’s biosynthetic machinery and metabolic building blocks. Advances in these technologies will also produce new, multifaceted tactics for landscaping cell surfaces. The potential for these techniques is tremendous. As a professional cell surface engineering services provider, Creative Biolabs is capable of providing customer-satisfied cell surface-based metabolic conjugation services to global clients.

Metabolic engineering for introducing unnatural functional groups into cell-surface glycans for the subsequent bioorthogonal reaction. (Bi, 2018)Fig.1 Metabolic engineering for introducing unnatural functional groups into cell-surface glycans for the subsequent bioorthogonal reaction. (Bi, 2018)

Introduction of Cell Surface-based Metabolic Conjugation

Much of enzyme-mediated labeling requires some aspects of time-intensive genetic engineering for tagged substrates. The continued pursuit of imparting orthogonality without the use of genetic manipulation has ignited the field of metabolic engineering. It is now possible to create new cell-surface epitopes via the metabolic incorporation of unnatural precursors into plasma membrane components. Oligosaccharides are particularly amenable to this type of metabolic engineering since the enzymes and transport proteins in certain biosynthetic pathways are tolerant of unnatural substrates. Cells will take up monosaccharide analogs from the extracellular environment and process them into cell-surface glycoconjugates. In contrast, the pathways for protein biosynthesis are more restrictive, and only the most conservatively modified amino acids are accepted.

Methods and Applications

Tumor targeted metabolic labeling. Fig.2 Tumor targeted metabolic labeling. (Abbina, 2018)

Services at Creative Biolabs

Committed to cell surface conjugation research over years, Creative Biolabs has accumulated strong foundations such as advanced facilities, the latest technologies, and a team of professional experts. With reliable foundations and extensive experience accumulated step-by-step during practice, we are confident in providing every customer with satisfying cell surface-based metabolic conjugation services.

As an industry-leading cell surface engineering services provider, Creative Biolabs is your best partner. If you are interested in cell surface-based metabolic conjugation services or other cell surface engineering services, please feel free to contact us for more information.

References

  1. Abbina, S.; et al. Surface engineering for cell-based therapies: Techniques for manipulating mammalian cell surfaces. ACS Biomater Sci Eng. 2018, 4(11): 3658-3677.
  2. Bi, X.; et al. Chemical and enzymatic strategies for bacterial and mammalian cell surface engineering. Chemistry. 2018, 24(32): 8042-8050.

All services are only provided for research purposes and Not for clinical use.

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