DNA-protein conjugates have recently been explored for many biomedical applications, including the delivery of antibodies and enzymes and cancer therapy. CreatBiolabsive has been a long-term expert in the field of cell conjugate. Due to the advanced technology platforms and extensive experience for DNA-protein conjugate, our seasoned scientists have set up one-stop procedures to assist your DNA-protein conjugation demands with the highest efficiency and the best quality.

DNA-Protein Conjugate

DNA and protein are the most critical two classes of biomacromolecules forming the basis of life. Given the high selectivity of DNA molecules, it is desirable to combine them with the functional versatility of proteins. The conjugation of the two enables a combination of molecular recognition, enzymatic catalysis, and Watson-Crick hybridization properties. DNA-protein conjugates, with their combined properties, have a broad range of applications, such as in high-performance biomedical diagnostic assays, therapeutic agents, fundamental research on molecular recognition, and DNA nanostructures synthesis. Moreover, these conjugates have been used as carriers in drug-delivery systems to deliver enzymes or available drugs for disease treatments.

Fig.1 Application of DNA-protein conjugate. (Zhao, 2020)

One-stop DNA-Protein Conjugate Service

To provide best-in-class DNA-protein conjugate services, Creative Biolabs brings together the most renowned DNA-protein conjugate experts and long-term experience in cell-based modification. We have developed a non-genetic cell-surface engineering technology that will quickly and stably couple DNA to the interested proteins.

• DNA and Protein Preparation
  Using our most advanced DNA and protein production platform, DNA and protein used for conjugation will be custom synthesized by Creative Biolabs according to customers' specific research needs.
• Design and Synthesis of DNA-Protein Conjugates
  The development of a highly efficient, robust, and reliable DNA-protein conjugation method is significant for its applications. Based on our CellFace™ conjugate technology platform, Creative Biolabs offers various ways for the chemical coupling of synthetic DNA with proteins of interest, which rely on either covalent or noncovalent strategies. Our synthesis process is generally rapid and easy-to-use, with the protein activity well preserved.
  
  • Covalent strategies for DNA-protein
    (1) Disulfide and maleimide coupling
    (2) Azide-alkyne cycloaddition
  • Noncovalent strategies for DNA-protein conjugate
    (1) Biotin-streptavidin interaction
    (2) Ni-NTA-His6 interaction
    (3) Antibody-antigen interaction
    (4) Aptamers-protein interaction
    (5) Reconstitution of apoenzymes

Creative Biolabs offers synthetic DNA-protein conjugates generated by either covalent or non-covalent coupling chemistry, which are versatile molecular tools applicable in bioanalytical and synthetic chemical procedures. Moreover, Creative Biolabs also provides other state-of-art strategies, such as site-specific labeling and enzymatic ligation methods, to meet complex conjugate design requirements to generate conjugate products with better performance.

To date, remarkable progress has been made in the field of DNA-protein conjugates for a broad range of diagnostic, therapeutic, and nano-assembly applications. Creative Biolabs is dedicated to delivering our customers high-quality DNA-protein conjugate services thanks to highly specialized staff and an advanced conjugation platform. If you have any questions, please do not hesitate to contact us for more information.

References

1. Zhao, D.; et al. Engineering Functional DNA-Protein Conjugates for Biosensing, Biomedical, and Nanoassembly Applications. Topics in Current Chemistry. 2020, 378, pp.1-42.
2. Niemeyer, C.M. Semisynthetic DNA–protein conjugates for biosensing and nanofabrication. Angewandte Chemie International Edition. 2010, 49(7), pp.1200-1216.