Lipid-DNA Conjugate Service

Lipid-DNA conjugates are robust molecular-engineering materials for various applications ranging from biosensors to biomedicine. Based on our CellFace™ conjugate technology platform, Creative Biolabs now offers a one-stop lipid-DNA conjugate service to meet all our global customers' specific requirements.

Better understanding and modulation of the compositions and functions of cell membranes is critical for regulating cell signaling and interactions. Among various strategies developed for cell membrane study and regulation, lipid-DNA conjugates are powerful tools with great potentials.

Lipid-DNA Conjugates

Lipid-DNA conjugates are amphiphilic macromolecules combining the cell membrane insertion capability of lipids with the properties of DNAs in precise hybridization and programmability. Interestingly, by chemically conjugating hydrophobic lipid molecules and DNAs together, several attractive features for cellular applications have been exhibited, including (1) spontaneous, rapid, and efficient membrane insertion after simple incubation; (2) universality toward different cell types; and (3) versatile functionality thanks to the simple DNA conjugation strategies. Taking advantage of lipid-DNA conjugates, differently functional moieties can be efficiently engineered onto live cell membranes without affecting natural cellular behavior.

Fig.1 Application of lipid-DNA conjugates. (Bagheri, 2019)

Our One-stop Lipid-DNA Conjugate Service

• Lipid and DNA Preparation

Creative Biolabs has extensive expertise in the design and synthesis of lipid and DNA. Our scientists can cost-effectively provide synthesized DNA and lipid to further improve the cellular and in vivo biostability of lipid-DNA conjugates.

• Lipid-DNA Conjugation

Based on our CellFace™ conjugate technology platform, a diverse range of conjugation approaches is available at Creative Biolabs to achieve a custom synthesis of lipid-DNA conjugates with designed DNA sequences. These one-stop strategies developed by our scientist team are a fast, simple, and cost-effective technique for cell surface engineering.

The preparation of lipid-DNA conjugates can be performed either before or after solid-phase DNA synthesis. In the pre-synthetic approach, lipid phosphoramidites are first synthesized and inserted into desired DNA sequences during solid-phase DNA synthesis. In the post-synthetic method, lipid moiety modification is achieved after the complete synthesis and purification of the DNA sequence. Our well-developed lipid-DNA conjugation strategies have paved the way for the emerging biological applications of lipid-DNA conjugates.

Fig.2 Strategy of lipid-DNA conjugates. (Li, 2020)

• Analysis and Characterization
  Consisting of a specific DNA for target recognition and a hydrophobic tail for anchoring membranes or driving self-assembly, lipid-DNA conjugates have unique properties owing to their two-part structures. Creative Biolabs provides a full set of lipid-DNA conjugates characterization services, such as:
  1. Recognition ability analysis
  2. Stability analysis

  These are the two main properties and prerequisites for lipid-DNA conjugates to function well in different applications, including bioanalytical and biomedical applications.

Synthetic lipid-DNA conjugates have shown great potential in studying membrane physiology and in regulating cell functions. Based on our CellFace™ conjugate technology platform, Creative Biolabs offers lipid-DNA conjugates service to enable this living cell membrane modification for many applications. If you are interested in our services, please contact us for more information.

References

1. Bagheri, Y.; et al. Lipid-DNA conjugates for cell membrane modification, analysis, and regulation. Supramolecular Chemistry. 2019, 31(8), pp.532-544.
2. Li, X.; et al. Lipid-oligonucleotide conjugates for bioapplications. National Science Review. 2020, 7(12), pp.1933-1953.